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Pichia Protocols
This book focuses on recent developments of Pichia pastoris as a recombinant protein production system. Highlighted topics include a discussion on the use of fermentors to grow Pichia pastoris, information on the O- and N-linked glycosylation, methods for labeling Pichia pastoris expressed proteins for structural studies, and the introduction of mutations in Pichia pastoris genes by the methods of restriction enzyme-mediated integration (REMI). Each chapter presents cutting-edge and cornerstone protocols for utilizing P. pastoris as a model recomibinant protein production system. This volume fully updates and expands upon the first edition.
Eicosanoid Protocols
In Eicosanoid Protocols, Elias A. Lianos and a panel of hands-on experts present cutting-edge methods for the study of eicosanoids, including prostaglandins, thromboxanes, and leukotrienes. The readily reproducible methods described hereconcentrate on studying the regulation of expression and function of enzymes, particularly cyclooxygenase (and its two isoforms), phospholipase A2, and lipoxygenases involved in the synthesis of established eicosanoids. Additional chapters are devoted to the characterization and distribution of the thromboxane A2 receptor in tissues and the biological roles of novel eicosanoids. Timely and authoritative, the methods in this book will help their users in exploring the pathobiology of inflammation. Eicosanoid Protocols offers new and established researchers powerful, state-of-the-art tools to probe the regulation and function of eicosanoids.
Guide to Protein Purification
Guide to Protein Purification, Second Edition provides a complete update to existing methods in the field, reflecting the enormous advances made in the last two decades. In particular, proteomics, mass spectrometry, and DNA technology have revolutionized the field since the first edition's publication but through all of the advancements, the purification of proteins is still an indispensable first step in understanding their function. This volume examines the most reliable, robust methods for researchers in biochemistry, molecular and cell biology, genetics, pharmacology and biotechnology and sets a standard for best practices in the field. It relates how these traditional and new cutting-ed...
2-D Proteome Analysis Protocols
With the completion of sequencing projects and the advancement of a- lytical tools for protein identification, proteomics—the study of the expressed part of the genome—has become a major region of the burgeoning field of functional genomics. High-resolution 2-D gels can reveal virtually all p- teins present in a cell or tissue at any given time, including posttranslationally modified proteins. Changes in the expression and structure of most cellular proteins caused by differentiation or external stimuli can be displayed and eventually identified using 2-D protein gels. 2-D Proteome Analysis Protocols covers all aspects of the use of 2-D protein electrophoresis for the analysis of biologi...
Stem Cell Assays
The scope for improving health care using stem cell therapies is thrilling, but has considerable technical challenges and methodological constraints that need to be addressed. Keeping with the tradition of Humana Press to bring these developments to the forefront in a timely manner, this book presents scientific advances in stem cell methods for a wider use by novice and expert scientists, through the series of Methods in Molecular Biology.
Innate Immunity
Immunologists today are interested in all of the diverse cell-types involved in host defense and have a deeper appreciation of the importance of innate immune mechanisms as a first line of protection against pathogens. This volume thus discusses the isolation and functional characterization of cells involved in innate immunity in mouse and man, including mast cells and eosinophils. Other focuses include natural killer cells, methods in statistics, in vivo imaging, genome engineering, and mutagenesis and culture that are adapted to the study of innate immunity in these hosts. These are complemented with a series of chapters dealing with alternative models: plants, worms, mosquitoes, flies, and fish. Together, these approaches and models are being used to dissect the complex interplay between hosts and pathogens and contribute to developing strategies to help fight infection. With chapters written by experts on the cutting-edge of this technology, Innate Immunity is an essential reference for immunologists, histologists, geneticists, and molecular biologists.
Gene Function Analysis
With the advent of high-throughput technologies following completion of the human genome project and similar projects, the number of genes of interest has expanded and the traditional methods for gene function analysis cannot achieve the throughput necessary for large-scale exploration. This book brings together a number of recently developed techniques for looking at gene function, including computational, biochemical and biological methods and protocols.
Avidin-Biotin Interactions
This book is specifically about the application of the extremely powerful interaction between the protein avidin or its homologues and the vitamin biotin and some of its homologues. With excellent descriptions of laboratory protocols written by expert researchers, this volume is equally perfect for the student or the professional laboratory scientist.
Plant Hormone Protocols
Established investigators from around the world describe in step-by-step detail their best techniques for the study of plant hormones and their regulatory activities. These state-of-the-art methods include contemporary approaches to identifying the biosynthetic pathways of plant hormones, monitoring their levels, characterizing the receptors with which they interact, and analyzing the signaling systems by which they exert their effects. Comprehensive and fully detailed for reproducible laboratory success, Plant Hormone Protocols offers plant biologists an indispensable compendium of today's most powerful methods and strategies to studying plant hormones, their regulation, and their activities.
Integrin Protocols
In Integrin Protocols, Anthony Howlett and a distinguished panel of experimentalists describe in detail a series of cutting-edge methods for dissecting the role of integrins in biological processes. This wide-ranging collection includes protocols for the analysis of integrin expression-at both the RNA and protein levels-and for elucidating the functional properties of integrins, including those at the cellular level. Each method provides step-by-step instructions for easy reproducibility, along with extensive notes about potential pitfalls, and tips on how to avoid failure. The emphasis is always on the practical steps necessary for experimental success and robust results. Offering powerful tools for understanding how integrins regulate cell growth, differentiation, migration, invasion, angiogenesis, and apoptosis, as well as how abnormalities of integrin expression and function may be implicated in various pathologic conditions, Integrin Protocols constitutes a gold-standard collection of techniques for both new and experienced investigators of the molecular and cellular basis of cardiovascular disease, inflammatory disorders, and cancer.
