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Hematologic Malignancies
The aim of Hematologic Malignancies: Methods and Techniques is to review those methods most useful for the diagnosis and subsequent mana- ment of hematologic malignancies. The scope of coverage is intentionally broad, ranging from routine procedures to highly sophisticated methods not currently offered by most clinical laboratories. The latter methods were selected especially to bring into focus recent advances in molecular biology that, since they provide us with strong tools for assessing the outcome of upcoming therapeutic modalities intent on disease eradication, are expected to impact the future diagnosis and management of these diseases. Thus, the c- mon thread among all chapters is cl...
Molecular Analysis of Cancer
Over the past 20 years, technological advances in molecular biology have proven invaluable to the understanding of the pathogenesis of human cancer. The application of molecular technology to the study of cancer has not only led to advances in tumor diagnosis, but has also provided markers for the assessment of prognosis and disease progression. The aim of Molecular Ana- sis of Cancer is to provide a comprehensive collection of the most up-to-date techniques for the detection of molecular changes in human cancer. Leading researchers in the field have contributed chapters detailing practical pro- dures for a wide range of state-of-the-art techniques. Molecular Analysis of Cancer includes chap...
Lung Cancer
The Methods in Molecular Medicine series is intended as a resource for both novice and experienced investigators attempting to diversify their tech- logical base in research. Lung Cancer: Volume 1: Molecular Pathology Me- ods and Reviews presents an overview of the current status of assays employed to detect and characterize the multitude of pathologies that contribute to the development of this deadly disease. As with all volumes in the Methods in Molecular Medicine series, the reader should find that each methods-based chapter provides clear instructions for the performance of various protocols, supplemented by additional technical notes that provide valuable insight. These notes are desig...
Lung Macrophages in Health and Disease
"This Ebook is edited by Sandra Hodge, a recognized expert in the field of macrophage dysfunction in chronic lung disease. The book consists of 8 chapters which provide a full coverage of macrophage function in both healthy and chronically diseased lungs, "
Wound Healing
During the past decade, significant progress in molecular and cellular te- niques has greatly advanced our understanding of the wound healing p- cess. Many of these new techniques have been utilized in the context of more classic models of wound healing. The combination of new and classic approaches has allowed scientists to make exciting discoveries in the field of tissue repair, resulting in an explosion of information about the healing p- cess. Importantly, these new findings have great relevance beyond wound healing itself. The injury repair process cuts across many disciplines, exte- ing to such broad fields as cancer, inflammation, and atherosclerosis. The relevance of the field to the...
Gene Therapy Protocols
Efforts in gene therapy have grown dramatically in recent years. Basic research as well as clinical activity have made exciting progress and are beg- ning to offer renewed hope that gene therapy may be able to deliver novel approaches for the treatment of inherited as well as such acquired diseases as cardiovascular disease and cancer. With the sequencing of the human genome complete, we now have a comprehensive catalog of genes that further expands the potential role of gene therapy into such new fields as tissue engineering. Central to gene therapy is the process of gene transfer; thus, advances in the technology of gene transfer are at the heart of this field’s progress. Numerous technologies, based on a variety of methods (e.g., viral-mediated, physical/ chemical), have been developed to achieve gene transfer. Some of the earliest methods, such as recombinant retroviruses, are still widely used, have undergone significant improvements, and have given rise to new vectors based on lentiviruses.
Appendix to the Journals of the Senate and Assembly ... of the Legislature of the State of California ...
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Antibiotic Resistance Methods and Protocols
At a time of rising concern about drug resistance and falling output of new antibacterial compounds, antibiotic research has once again returned to the forefront of medical science. In Antibiotic Resistance: Methods and Protocols, Stephen Gillespie and a panel of leading clinical and diagnostic microbiologists describe a series of detailed molecular and physical methods designed to study the growing problem of antibiotic resistance, as well as facilitate new antibiotic research programs for its effective redress. The techniques range widely from those that provide rapid diagnosis via DNA amplification and phage display, to those for plotting the transmission of resistant organisms and invest...
Dendritic Cell Protocols
Dendritic Cell Protocols provides chapter and verse for many useful practical approaches to the art of studying dendritic cells. The book gives information on the usual techniques for derivation of human dendritic cells from precursor stem cells, such as monocytes. In addition it provides data on the difficult tasks of isolating dendritic cells directly from different tissues; whether dendritic cells from precursor cells or from tissues of mouse or human are required, this book contributes practical information. The last section of the book is devoted to functional aspects of dendritic cells ranging from inf- mation relevant to cell migration to antigen uptake and T cell stimulation. But wha...
