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Methods of Enzymatic Analysis, Volume 4 reviews developments in the use of enzymes as tools in analytical biochemistry, including advances in assay techniques. It discusses the principles and methods for the elucidation of structures of enzymes, such as peptides, proteins, amino acids, fatty acid metabolites, lipids, steroids, nucleic acids, purines, pyrimidines, nucleosides, and coenzymes. It also considers the isolation and characterization of active centers in enzymes. This volume is divided into four parts, each discussing a group of enzymes and their determination. Part I focuses on proteins, peptides, and amino acids including amines and amides. Part II is concerned with fatty acid met...
This twelve volume series is a useful reference manual for work in the laboratory. Seven hundred authors from 29 countries describe the methods of enzymatic analysis in a way that corresponds to the various steps undertaken in laboratory routine. Designed for use in the laboratory rather than in the library, the third edition takes into account recent changes in symbolism, nomenclature, & enzyme classification. The series provides information on the precision of measurement, the sensitivity & limitation of a method, & on possible alternatives. Annotations by the authors convey their personal experience by including useful hints, remarks & experimental tricks to make the method truly workable.
Enzyme nomenclature. Structure of enzymes. Enzymes and molecular biology. Catalytic activity of enzymes. Quality evaluation of enzyme preparations. General production methods: fermentation, isolation and purification, immobilization. Industrial uses of enzymes: survey of industrial enzymes, enzymes in starch processing and baking, glucose isomerization, meat processing, dairy products, processing of fruit, vegetables, and wine, hidrolysis of protein, fat, and cellusose, and inversion of sucrose. Enzymes in organic synthesis. Enzymes in analysius and medicine. Enzymes in genetic engineering. Modifying enzymes.
Methods of Enzymatic Analysis, Volume 2 reviews developments in the determination of enzyme activity, including advances in assay techniques. It discusses the principles on which measurements of enzymes are based, with each chapter including equations and each method consisting of the pipetting protocol. This volume is divided into four parts, each discussing a group of enzymes and their determination. Part I focuses on oxidoreductases, such as sorbitol dehydrogenase, lactate dehydrogenase, malate dehydrogenase, isocitrate dehydrogenase, 6-phosphogluconate dehydrogenase, xanthine oxidase, and glutamate dehydrogenase. Part II is concerned with transferases ranging from ornithine carbamoyltran...