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Tag-based approaches were originally designed to increase the throughput of capillary sequencing, where concatemers of short sequences were first used in expression profiling. New Next Generation Sequencing methods largely extended the use of tag-based approaches as the tag lengths perfectly match with the short read length of highly parallel sequencing reactions. Tag-based approaches will maintain their important role in life and biomedical science, because longer read lengths are often not required to obtain meaningful data for many applications. Whereas genome re-sequencing and de novo sequencing will benefit from ever more powerful sequencing methods, analytical applications can be perfo...
An eye-opening game-changer of a book that sheds new light on how horses learn, think, perceive, and perform, and explains how to work with the horse’s brain instead of against it. In this illuminating book, brain scientist and horsewoman Janet Jones describes human and equine brains working together. Using plain language, she explores the differences and similarities between equine and human ways of negotiating the world. Mental abilities—like seeing, learning, fearing, trusting, and focusing—are discussed from both human and horse perspectives. Throughout, true stories of horses and handlers attempting to understand each other—sometimes successfully, sometimes not—help to illustr...
Prior to 1862, when the Department of Agriculture was established, the report on agriculture was prepared and published by the Commissioner of Patents, and forms volume or part of volume, of his annual reports, the first being that of 1840. Cf. Checklist of public documents ... Washington, 1895, p. 148.
Matthew is the third volume in the forty-volume Brazos Theological Commentary on the Bible. This commentary, like each in the series, is designed to serve the church--through aid in preaching, teaching, study groups, and so forth--and demonstrate the continuing intellectual and practical viability of theological interpretation of the Bible.
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A masterful poetic exploration of the impact of Matthew Shepard’s murder on the world. On the night of October 6, 1998, a gay twenty-one-year-old college student named Matthew Shepard was kidnapped from a Wyoming bar by two young men, savagely beaten, tied to a remote fence, and left to die. Gay Awareness Week was beginning at the University of Wyoming, and the keynote speaker was Lesléa Newman, discussing her book Heather Has Two Mommies. Shaken, the author addressed the large audience that gathered, but she remained haunted by Matthew’s murder. October Mourning, a novel in verse, is her deeply felt response to the events of that tragic day. Using her poetic imagination, the author creates fictitious monologues from various points of view, including the fence Matthew was tied to, the stars that watched over him, the deer that kept him company, and Matthew himself. More than a decade later, this stunning cycle of sixty-eight poems serves as an illumination for readers too young to remember, and as a powerful, enduring tribute to Matthew Shepard’s life. Back matter includes an epilogue, an afterword, explanations of poetic forms, and resources.