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Bioprocess Intensification
Application of Process Intensification (PI) presents a set of radically innovative principles in process and equipment design, which can bring significant benefits in terms of process efficiency, capital and operating expenses, quality, process safety, and sustainability. Typical approaches in bioprocess intensification are the reduction of the number of production steps, continuous processing, integrated processes, and alternative energy inputs.
Microfluidics in Biotechnology
This new volume introduces the applications of microfluidic systems to facilitate biotechnological and biomedical processes. It provides an overview on cutting-edge technologies, summarizes traditional and modern fabrication methods and highlights recent advances regarding the application of lab-on-a-chip (LoC) systems for bioanalytical purposes. This book is ideal for research scientists and students interested at the cross-section between biotechnology, chemistry and chemical engineering.
Numerical Characterization of Mechanical Stress and Flow Patterns in Stirred Tank Bioreactors
Das Ziel der Arbeit bestand darin, die lokalen und globalen mechanischen Beanspruchungsparameter in einem Rührkesselbioreaktor mit Hilfe der numerischen Strömungssimulation (CFD) nachzubilden und die Ergebnisse mit experimentellen Daten der Pellet-kultivierung bzgl. Fluiddynamik und Rheolgie des rekombinanten Stammes A. niger SKAn1015 zu validieren. Ausgehend von der einphasigen Strömungssimulation eines 2L-Bioreaktors werden zunächst Simultionsdaten über Strömungsgeschwindigkeit und mechanische Beanspruchung diskutiert. Aßerdem erfolgt eine Validierung der einphasigen Simulationsdaten mit der Particle Image Velocimetry. In weiteren Untersuchungen wird die Strömungssimulation auch au...
Micro- and Macroparticle enhanced cultivation of filamentous Lentzea aerocolonigenes for increased rebeccamycin production
The filamentous actinomycete Lentzea aerocolonigenes produces the antitumor antibiotic rebeccamycin. However, the complex morphology of actinomycetes leads to challenges during the cultivation often accompanied by low product titers. In the recent past, the to date low rebeccamycin titers were increased by particle addition to cultivations of L. aerocolonigenes. In this thesis the addition of micro-, macro- and adsorbent particles to cultivations of L. aerocolonigenes were investigated in more detail. Furthermore, the scale-up to a bubble-free bioreactor was conducted. The addition of glass microparticles (x50 = 7.9 μm, 10 g L-1) to shake flask cultivations increased the rebeccamycin titer ...
Production of labyrinthopeptin A1 with Actinomadura namibiensis
The filamentous actinomycete Actinomadura namibiensis is the only known producer of labyrinthopeptins, a class of ribosomally synthesized and posttranslationally modified peptides (RiPPs) displaying highly attractive bioactive properties. In order to increase the labyrinthopeptin A1 productivity in shaking flask cultivations of A. namibiensis, a new cultivation method called salt-enhanced cultivation was used. Compared to the unsupplemented control, labyrinthopeptin A1 productivity was enhanced the most by addition of 50 mM (NH4)2SO4, reaching a 7-fold higher yield of 325 mg L-1 within 10 cultivation days. Salt-enhanced cultivation affected growth and product formation mechanisms, cell morph...
Dispersity, Structure and Phase Changes of Proteins and Bio Agglomerates in Biotechnological Processes
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Pathway, Genetic and Process Engineering of Microbes for Biopolymer Synthesis
Professor Bruce Ramsay holds a patent for a method of synthesising medium chain length polyhydroxyalkanoate. All other Guest Editors declare no competing interests with regards to the Research Topic subject.
Characterisation of the Mechanical Properties of Heat-Induced Protein Deposits in Immersed Cleaning Systems
During heat treatment in dairy production, the rapid formation of heat-induced fouling deposits on the plant surface leads to reduced efficiency of heat transfer. Therefore, a regular cleaning process is required to soften the heat-induced protein deposits and then remove them from the plant surface. The mechanical property of the deposits is one of the key issues of the cleaning mechanisms since the non-fractured behaviour dominates the deformation of the fouling layer and the failure behaviour has a great impact on the cohesive removal of fouling deposits. Considering the complicated geometry of fouling deposits and their irregular distribution, indentation experiments were carried out on ...
Comprehensive Biotechnology
Comprehensive Biotechnology, Third Edition, Six Volume Set unifies, in a single source, a huge amount of information in this growing field. The book covers scientific fundamentals, along with engineering considerations and applications in industry, agriculture, medicine, the environment and socio-economics, including the related government regulatory overviews. This new edition builds on the solid basis provided by previous editions, incorporating all recent advances in the field since the second edition was published in 2011. Offers researchers a one-stop shop for information on the subject of biotechnology Provides in-depth treatment of relevant topics from recognized authorities, including the contributions of a Nobel laureate Presents the perspective of researchers in different fields, such as biochemistry, agriculture, engineering, biomedicine and environmental science
Dynamic and Fatigue Assessment of Heavy-Duty Engine Valves
This book provides findings on the simulation of the valve dynamic to the current technological standards. Above all, it delivers a simulation based and predictive approach on the fatigue strength assessment of four-stroke heavy-duty engine valves. The demand for more efficient combustion engines with fuel flexibility goes along with increasing component requirements regarding strength and durability, while the development costs should remain low. In this context, the present book focuses on the gas exchange valves of heavy-duty engines. Especially, the valves on the exhaust side have an increased risk of fatigue failure. The aim of this book is the generation of a predictive fatigue strengt...
