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The Protein Protocols Handbook
In The Protein Protocols Handbook, I have attempted to provide a cross-section of analytical techniques commonly used for proteins and peptides, thus providing a benehtop manual and guide both for those who are new to the protein chemistry laboratory and for those more established workers who wish to use a technique for the first time. We each, of course, have our own favorite, commonly used gel system, g- staining method, blotting method, and so on; I'm sure you will find yours here. H- ever, I have also described a variety of altematives for many of these techniques; though they may not be superior to the methods you commonly use, they may nev- theless be more appropriate in a particular situation. Only by knowing the range of techniques that are available to you, and the strengths and limitations of these te- niques, will you be able to choose the method that best suits your purpose.
Immunogenicity of Biopharmaceuticals
Immunogenicity of Biopharmaceuticals is the first book to comprehensively address the potential of an immune response to biopharmaceuticals. It is intended to give a broad overview of the current state-of-the-art regarding this subject. The chapters range from an overview of the immune system and factors that may trigger the immune system, via detection of antibodies and clinical implications, to various case examples and the regulatory view on immunogenicity.
Cytokine Cell Biology
Cytokine Cellular Biology focuses on cell biology techniques for studying cytokines, cytokine receptors, and cytokine driven processes. Assays for human B cell responses, leucocyte migration, haematopoietic growth factors, macrophage activation by cytokines, RIA, IRMA, and ELISA assays, and quantitative biological assays for cytokines are all covered in detail. There are also updated chapters on studying cytokine regulation of endothelial cells; the measurement of proliferative, cytostatic, and cytolytic activity of cytokines; and the development of antibodies to cytokines. In addition there is a new chapter on the use of flow ctyometry and intracellular fluorescent staining. Written by experts in the field, Cytokine Molecular Biology and Cytokine Cellular Biology form a comprehensive and essential guide to cytokine research.
Detection and Quantification of Antibodies to Biopharmaceuticals
The definitive book on the neutralization of recombinant biopharmaceuticals Recombinant biopharmaceuticals are an important tool for treating a range of illnesses; however, their efficacy can be severely impaired by their immunogenicity. When introduced into the body, these pharmaceuticals can cause the immune system to produce anti-drug antibodies (ADAs) that neutralize their effects. The first and only book to cover neutralization in connection with biopharmaceuticals and the measurement and application of neutralizing antibodies in modern medicine at any real length, Detection and Quantification of Antibodies to Biopharmaceuticals: Practical and Applied Considerations offers a comprehensi...
Immunochemical Protocols
This much anticipated second edition provides a user-friendly, up-to-date handbook of reliable immunochemical techniques optimized for molecular biologists. It covers the breadth of relevant established methods from protein blotting and immunoassays through to visualization of cellular antigens and in situ hybridization, each with their latest refinements. Protocols for the production and purification of important classes of immunochemical reagents are also provided, including "conventional" and recombinant antibodies, fusion proteins and their various conjugates. This book will open the door to a new generation of immunochemical reagents with exciting possibilities.
Training Secrets of the World's Greatest Footballers
'Incredibly well-researched and loaded with modern-day, high-tech football insights' – Tony Strudwick, Head of Performance, Wales national football team Professional football is more demanding than ever. Top internationals reach speeds of 36km/hr, run 12km each match and play up to 60 games each season. Sports scientists are now key figures at every top club, applying cutting-edge techniques to boost fitness, accelerate recovery and forge lean, mean, winning machines. This illuminating book uncovers the training and fuelling secrets of today's greatest footballers, drawing on access to the world's best clubs, including Barcelona, Manchester City, Manchester United, Liverpool, Bayern Munich, Paris Saint-Germain and many more. Why does Cristiano Ronaldo have his own cryotherapy chamber? Why does Paul Pogba wear custom-made compression socks? Why does Sergio Agüero altitude-train when returning from injury? From virtual-reality units to the omnipresence of GPS vests, taking in brain-training, innovative gear and performance nutrition along the way, you'll discover what it takes to reach the top of the game – and how to apply this knowledge to your own training.
Science and Soccer
Now in a fully revised and updated fourth edition, Science and Soccer is still the most comprehensive and accessible introduction to the physiology, biomechanics and psychology behind the world's most popular sport. Offering important guidance on how science translates into practice, the book examines every key facet of the sport, with a particular focus on the development of expert performers. The topics covered include: • anatomy, physiology, psychology; sociology and biomechanics; • principles of training; • nutrition; • physical and mental preparation; • playing surfaces and equipment; injury • decision-making and skill acquisition; • coaching and coach education; • performance analysis; • talent identification and youth development. Science and Soccer: Developing Elite Performers is a unique resource for students and academics working in sports science. It is essential reading for all professional support staff working in the game, including coaches at all levels, physiotherapists, conditioning specialists, performance analysts, club doctors and sport psychologists.
Stormsong
“Fantastical, thoughtful, and un-put-downable, I flew through Stormsong and want the third in the series NOW.” —Elizabeth Hoyt A Reader's Digest Top 25 Best Fantasy Series Ever Written After spinning an enthralling world in Witchmark, the winner of the World Fantasy Award for best novel that was praised as a “can't-miss debut” by Booklist, and as “thoroughly charming and deftly paced” by the New York Times, C. L. Polk continues the Kingston Cycle in Stormsong. Magical cabals, otherworldly avengers, and impossible love affairs conspire to create a book that refuses to be put down. Dame Grace Hensley helped her brother Miles undo the atrocity that stained her nation, but now she ...
Immune Aspects of Biopharmaceuticals and Nanomedicines
The enormous advances in the immunologic aspects of biotherapeutics and nanomedicines in the past two decades has necessitated an authoritative and comprehensive reference source that can be relied upon by immunologists, biomedical researchers, clinicians, pharmaceutical companies, regulators, venture capitalists, and policy makers alike. This text provides a thorough understanding of immunology, therapeutic potential, clinical applications, adverse reactions, and approaches to overcoming immunotoxicity of biotherapeutics and nanomedicines. It also tackles critical, yet often overlooked topics such as immune aspects of nano-bio interactions, current FDA regulatory guidances, complement activation-related pseudoallergy (CARPA), advances in nanovaccines, and immunogenicity testing of protein therapeutics.
Football Science and Performance Coaching
In recent decades, the sporting landscape has seen many changes, notably the inclusion and professionalization of sport science and coaching. With this change has come significant demand for applied research surrounding soccer science and performance coaching, leading to new theory and methodology across all aspects of the game. This book brings together the most fundamental components of soccer science and performance coaching through modern, integrated coaching science methods implemented by leading practitioners and researchers. The expertise included in this book provides a unique blend of modern, soccer-specific research trends with innovative coaching theory, implemented at an elite le...
