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This book provides a general introduction as well as a selected survey of key advances in the fascinating field of plant cell and tissue culture as a tool in biotechnology. After a detailed description of the various basic techniques employed in leading laboratories worldwide, follows an extended account of important applications in, for example, plant propagation, secondary metabolite production and gene technology. Additionally, some chapters are devoted to historical developments in this domain, metabolic aspects, nutrition, growth regulators, differentiation and the development of culture systems. The book will prove useful to both newcomers and specialists, and even “old hands” in tissue culture should find some challenging ideas to think about.
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Nature’s high biomass productivity is based on biological N2 fixation (BNF) and biodiversity (Benckiser, 1997; Benckiser and Schnell, 2007). Although N2 makes up almost 80% of the atmosphere’s volume living organisms need it in only small quantities, presumably due to the paucity of natural ways of transforming this recalcitrant dinitrogen into reactive compounds. N shortage is commonly the most important limiting factor in crop production. The synthesis of ammonium from nitrogen and hydrogen, the Haber–Bosch (H-B) process, invented more than 100 years ago, became the holy grail of synthetic inorganic chemistry and removed the most ubiquitous limit on crop yields. H-B opened the way fo...
This book provides essential molecular techniques and protocols for analyzing microbes that are useful for developing novel bio-chemicals, such as medicines, biofuels, and plant protection substances. The topics and techniques covered include: microbial diversity and composition; microorganisms in the food industry; mass cultivation of sebacinales; host-microbe interaction; targeted gene disruption; function-based metagenomics to reveal the rhizosphere microbiome; mycotoxin biosynthetic pathways; legume-rhizobium symbioses; multidrug transporters of yeast; drug-resistant bacteria; the fungal endophyte piriformospora indica; medicinal plants; arbuscular mycorrhizal fungi; biosurfactants in microbial enhanced oil recovery; and biocontrol of the soybean cyst nematode with root endophytic fungi; as well as microbe-mediated drought tolerance in plants.
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This thoroughly revised edition of the book demonstrates principle and instrumentation of each technique routinely used in biotechnology. Like the previous edition, the second edition also follows non-mathematical approach. Three aspects of each technique including principle, methodology with knowledge of different parts of an instrument; and applications have now been discussed in the text. For the beginners, the book will help in building a strong foundation, starting from the preparation of solutions, extraction, separation and analysis of biomolecules to the characterisation by spectroscopic methods—the full gamut of biological analysis. NEW TO THE SECOND EDITION • Incorporates two n...
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