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The Protein Protocols Handbook
The authors are commonly the techniques" originators, and each has demonstrated a hands-on mastery of the methods described, always fine-tuning them here for optimal productivity.
Medical BioMethods Handbook
John Walker and Ralph Rapley have collected a wide-ranging group of molecular and biochemical techniques that are the most frequently used in medical and clinical research, especially diagnostics. The authors-well-established investigators who run their own research programs and use the methods on a regular basis-outline the practical procedures for using them and describe a variety of pertinent applications. Among the technologies presented are southern and western blotting, electrophoresis, PCR, cDNA and protein microarrays, liquid chromatography, in situ hybridization, karyotyping, flow cytometry, bioinformatics, genomics, and ribotyping. The applications include assays for mutation detection, mRNA analysis, chromosome translocations, inborn errors of metabolism, protein therapeutics, and gene therapy.
The Proteomics Protocols Handbook
Hands-on researchers describe in step-by-step detail 73 proven laboratory methods and bioinformatics tools essential for analysis of the proteome. These cutting-edge techniques address such important tasks as sample preparation, 2D-PAGE, gel staining, mass spectrometry, and post-translational modification. There are also readily reproducible methods for protein expression profiling, identifying protein-protein interactions, and protein chip technology, as well as a range of newly developed methodologies for determining the structure and function of a protein. The bioinformatics tools include those for analyzing 2D-GEL patterns, protein modeling, and protein identification. All laboratory-based protocols follow the successful Methods in Molecular BiologyTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Molecular Biomethods Handbook
Recent advances in the biosciences have led to a range of powerful new technologies, particularly nucleic acid, protein and cell-based methodologies. The most recent insights have come to affect how scientists investigate and define cellular processes at the molecular level. This book expands upon the techniques included in the first edition, providing theory, outlines of practical procedures, and applications for a range of techniques. Written by a well-established panel of research scientists, the book provides an up-to-date collection of methods used regularly in the authors’ own research programs.
Vegan Gardener
Here is a mindful, gentle, yet powerful way of gardening The natural world is undergoing profound change, and our ecology and climate are in crisis. Gardens, whatever their size, are where we can take practical action. A compassionate, ethical and planet-friendly approach. Welcome nature into your garden, then tempt it to stay there. Reduce your gardening footprint and develop a self-sufficient 'closed loop' garden. How to make garden compost and leaf mould, and harness the power of home-grown green wood chips to inject new life into soil. Book jacket.
Visual Culture
This book is about the expanding realm of visual culture: in architecture, art, design, advertising, photography, film, television, video, theatre performance, computer imagery and virtual reality. It is also about Visual Culture Studies, a relatively new academic discipline, or rather range of disciplines, that scholars employ to analyse visual artefacts. Unlike many other texts on the same subject, it foregrounds the ‘visual’ and is systematic and accessible. Visual culture provides an overview of the subject that pays heed to the achievements of both traditional and new theory whilst directing the reader to a large body of literature via references and an extensive bibliography. Walker and Chaplin discuss the concepts of ‘the visual’ and of ‘culture’ as well as the field and origins of Visual Culture Studies; coping with theory; models of production and consumption; institutions; pleasure; the canon and concepts of value; visual literacy and poetics; modes of analysis; culture and commerce; and new technologies. This book is designed for those studying the history and theory of fine arts, design and the mass media.
Microscopy, Optical Spectroscopy, and Macroscopic Techniques
This is the second of three volumes of Methods in Molecular Biology that deal with Physical Methods of Analysis. The first of these, Spectroscopic Methods and Analyses dealt with NMR spec troscopy, mass spectrometry, and metalloprotein techniques, and the third will cover X-ray crystallographic methods. As with the first volume. Microscopy, Optical Spectroscopy, and Macroscopic Techniques is intended to provide a basic understand ing for the biochemist or biologist who needs to collaborate with spe cialists in applying the techniques of modern physical chemistry to biological macromolecules. The methods treated in this book fall into four groups. Part One covers microscopy, which aims to vis...
