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This book deals with the molecular mechanisms of membrane trafficking, a central eukaryotic cell biological process. In the post-genomic era many essential molecules involved in intracellular membrane/protein transport are emerging. A huge task now is to compile the molecular networks that govern these processes. Understanding of regulatory processes and participating molecules are likely to reveal global cellular regulatory circuits that couple membrane trafficking with other cellular functions. Such cell biological features are only starting to emerge. This book puts special emphasis on such mechanisms and processes. The contents discusses the role of coat proteins, tethering complexes, small rab GTPases, Sec1-family proteins and SNARE molecule phosphorylation in exocytosis, endocytosis and membrane fusion. In addition, the role of lipids in vesicle formation and membrane fusion, and some specialized cell biological denovo membrane generation processes are discussed.
As membrane trafficking research has expanded over the past thirty years, a remarkable convergence of information has been gained by using genetic approaches in yeast cells with biochemical approaches in mammalian cells. This book reflects these advances by devoting one section of the book to yeast cells and the other to mammalian cells, with each section providing both classic and cutting-edge techniques to study macromolecular transport across the membranes.
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This second edition volume expands on the previous edition with new and updated chapters on the latest developments in the study of yeast within the biotechnology field. The chapters in this book cover topics such as transformation protocols for genetic engineering of Saccaromyces cerevisiae and Komagataella spp.; an overview of selection markers, promoters, and strains used for metabolic engineering of S. cerevisiae, P. pastoris, and Z. bailii; the use of yeast in CRISPR/Cas9 technology; tools to study metabolic pathway in Yarrowia lypolitica; and a discussion on the “universal expression system” that is applied in a broad spectrum of fungal species. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Yeast Metabolic Engineering: Methods and Protocols, Second Edition is a valuable resource for researchers and scientists interested in learning more about this important and developing field.
This new Springer volume, which comes complete with a free DVD, is a comprehensive and detailed overview of the synapse with emphasis on the glutamatergic synapse. Most chapters relate the synapse’s functional aspects to its molecular mechanisms. This approach shows which mechanisms are characterized on both the functional and structural level and can thus be considered firmly established. It’s an important text for neuroscientists and disease-oriented clinicians in neurology.
MBC online publishes papers that describe and interpret results of original research conserning the molecular aspects of cell structure and function.
Aki Hintsa – Voittamisen anatomia -kirjan kirjoittajan teos kertoo, kuinka menestyt. Huippuvalmentaja Petteri Nykky teki häviäjistä voittajia keskittymällä tuloksen sijasta menestyksen perustan rakentamiseen. Tämä kirja kertoo, miten se tapahtui. Petteri Nykky on syystä saanut lempinimen Alkemisti: häviämään tottunut Suomen salibandymaajoukkue on voittanut hänen johdollaan kultaa jo kolmissa MM-kisoissa. Voitot toi Nykyn metodi, jossa ei keskitytä tulokseen vaan ihmisen kokonaisvaltaiseen kasvuun, sisäisen motivaation löytämiseen, yhteisen luottamuksen rakentamiseen ja ihmisen sisällä olevien esteiden poistamiseen. Olipa kyseessä urheilu, yritystoiminta tai ihmisenä ka...