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The study of plant-microbe associations by new techniques has significantly improved our understanding of the structure and specificity of the plant microbiome. Yet, microbiome function and the importance of the plant’s microbiome in the context of human and plant health are largely unexplored. Comparable with our human microbiome, millions of microbes inhabit plants, forming complex ecological communities that influence plant growth and health through its collective metabolic activities and host interactions. Viewing the microbiota from an ecological perspective can provide insight into how to promote plant health and stress tolerance of their hosts or how to adapt to a changing climate b...
This Research Topic is dedicated In Memoriam of Dr. Nicola Sante Iacobellis who contributed to the conception of this article collection † This Research Topic collects the selected contributions to the 14th International Conference on Plant Pathogenic Bacteria (14th ICPPB), “The Impact of Plant Pathogenic Bacteria on Global Plant Health”, which was held in Assisi (Italy) from July 3 to 8, 2022. Occurrence of bacterial disease in plant is the result of complex interaction between host, bacterium and environment. The mechanisms by which bacteria cause shifts in the biochemical and physiological processes required for the plant life cycle as well as the mechanisms by which hosts prevent or respond or defend against attack by bacteria are the central themes of this Research Topic.
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This volume provides methods, protocols, and reviews that are useful for new and experienced plant microbiome researchers. Chapters guide readers through the investigation of microbiomes associated with seeds, sampling microbiomes from plant compartments and tissues, culture-based methods, culture-independent metabarcoding methods, methods to obtain DNA and perform metabarcoding, protocols to block PCR amplification from the plant host, qPCR-based methods, editing of specific genes in Bacillus genomes, and Streptomycetes and plant microbial indicators. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, The Plant Microbiome: Methods and Protocols aims to ensure successful results in the further study of this vital field.