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Molecular Biomethods Handbook
An authoritative team of investigators illuminate the core bioanalytical techniques used every day in their own laboratories, and laboratories throughout the world. These highly experienced scientists fully explain both the theory behind, and the application of, these key techniques, and include extensive references for those seeking detailed laboratory protocols. The techniques covered range from the extraction, separation, detection, and characterization of nucleic acids to gene cloning and library production, mapping, expression, transgenesis, differential display, and DNA profiling, to name a few. Numerous key protein methods, as well as support and related techniques, are also included. The goal is to provide established scientists and novices who are new to these techniques with a deeper understanding of the widest variety of biotechniques and their applications.
Compassionate Coaching
This surprisingly generous book maps out an incredibly effective, easy-to-follow framework for guiding you through the process of turning your dreams into reality. It also adds valuable learning tools and provides essential back-up material for coaching clients. The carefully designed format leads you gently from creating a compelling outcome, increasing your motivation to achieve it, appreciating your gifts, becoming more aligned with your heart, and then clearing whatever holds you back. Using lots of real life stories to illustrate each topic, this book can help you manifest miracles happening in your life.
Biotechnology of Major Cereals
Biotechnology of Major Cereals will focus on the recent advances and future prospects in cereal biotechnology. The first part of the book will cover the world’s major cereals and focus on new developments and trends. The second part will be technology rather than species-led, detailing fundamental developments in technologies and significant target traits.
Physiological, biochemical and molecular approaches in response to abiotic stresses in plants
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Plant Genome Editing with CRISPR Systems
This volume provides readers with wide-ranging coverage of CRISPR systems and their applications in various plant species. The chapters in this book discuss topics such as plant DNA repair and genome editing; analysis of CRISPR-induced mutations; multiplexed CRISPR/Cas9 systems; CRISPR-Cas12a (Cpf1) editing systems; and non-agrobacterium based CRISPR delivery systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and thorough, Plant Genome Editing with CRISPR Systems: Methods and Protocols is a valuable resource for any researcher interested in learning about and using CRISPR systems in plants.
Plant Biotechnology: Progress in Genomic Era
Refinement in sequencing technologies and potential of genomic research resulted in meteoric growth of biological information such as sequences of DNA, RNA and protein requiring databases for efficient storage, management and retrieval of the biological information. Also, computational algorithms for analysis of these colossal data became a vital aspect of biological sciences. The work aims to show the process of turning bioscience innovation into companies and products, covering the basic science, the translation of science into technology. Due to rapid developments, there seems to be no basic difference between the pharmaceutical industry and the biotechnological industry. However, approve...
Genome Editing
Over the last few decades, various techniques have been developed to alter the properties of plants and animals. While the targeted transfer of recombinant DNA into crop plants remains a valuable tool to achieve a desirable breeding outcome, integration of transgenes into the host genome has been random, which in part, leads to reduced acceptance of GMOs by the general population in some parts of the world. Likewise, methods of induced mutagenesis, such as TILLING, have the disadvantage that many mutations are induced per plant, which has to be removed again by expensive backcrossing. Advances in genome sequencing have provided more and more information on differences between susceptible and...
Population, Agriculture, and Biodiversity
This timely collection of 15 original essays written by expert scientists the world over addresses the relationships between human population growth, the need to increase food supplies to feed the world population, and the chances for avoiding the extinction of a major proportion of the world's plant and animal species that collectively makes our survival on Earth possible. These relationships are highly intertwined, and changes in each of them steadily decrease humankind’s chances to achieve environmental stability on our fragile planet. The world population is projected to be nine to ten billion by 2050, signaling the need to increase world food production by more than 70 percent on the same amount of land currently under production—and this without further damaging our fragile environment. The essays in this collection, written by experts for laypersons, present the problems we face with clarity and assess our prospects for solving them, calling for action but holding out viable solutions.
GMOs Decoded
The debate over genetically modified organisms: health and safety concerns, environmental impact, and scientific opinions. Since they were introduced to the market in the late 1990s, GMOs (genetically modified organisms, including genetically modified crops), have been subject to a barrage of criticism. Agriculture has welcomed this new technology, but public opposition has been loud and scientific opinion mixed. In GMOs Decoded, Sheldon Krimsky examines the controversies over GMOs—health and safety concerns, environmental issues, the implications for world hunger, and the scientific consensus (or lack of one). He explores the viewpoints of a range of GMO skeptics, from public advocacy gro...
Agrobacterium Protocols
Agrobacterium tumefaciens is a soil bacterium that for more than a century has been known as a pathogen causing the plant crown gall disease. Unlike many other pathogens, Agrobacterium has the ability to deliver DNA to plant cells and permanently alter the plant genome. The discovery of this unique feature 30 years ago has provided plant scientists with a powerful tool to genetically transform plants for both basic research purposes and for agricultural development. Compared to physical transformation methods such as particle bomba- ment or electroporation, Agrobacterium-mediated DNA delivery has a number of advantages. One of the features is its propensity to generate a single or a low copy...
